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Supports: TIFF, TIF
TIFF is a still-image container — high bit-depth, often lossless, and able to chain dozens or thousands of frames via linked Image File Directories (IFDs). AVI is Microsoft's 1992 video container, still the lingua franca for Windows-native players, legacy lab software, and frame-accurate analysis tools. Turning a TIFF sequence into AVI is how you go from "a folder of stills" or a multi-page scan to something you can scrub, share, and embed.
If you need the reverse direction or a more modern container, see AVI to MP4 or AVI to TIFF for frame extraction.
| Property | TIFF | AVI |
|---|---|---|
| Year introduced | 1986 (Aldus) | 1992 (Microsoft, Video for Windows) |
| Type | Still-image container (multi-page via IFD chain) | Audio-video container (RIFF-based) |
| Max file size | 4 GB (standard); 18 PB (BigTIFF, 64-bit offsets) | 1 GB (AVI 1.0 practical) / 4 GB+ (AVI 2.0 OpenDML, 1996) |
| Compression | None, LZW, Deflate/ZIP, JPEG, ZSTD, WebP, PackBits, CCITT G4 | Codec-dependent (MPEG-4, Xvid, DivX, MJPEG, H.264, uncompressed) |
| Bit depth | 1, 8, 16, 32, 64 bits per channel | 8 bits per channel (typical); 10/12-bit via specific codecs |
| Frame timing | None (stills only) | Per-frame timestamps and frame rate |
| Audio support | No | Yes (PCM, MP3, AC3, AAC, etc.) |
| Best for | Archival masters, scientific imaging, prepress | Frame-accurate Windows playback, legacy NLE ingest |
| Codec | Compression | Best for | Notes |
|---|---|---|---|
| MJPEG | Intra-frame JPEG per frame | Microscopy, machine vision, frame-accurate scrubbing | Each frame is independently decodable; large files but trivial to edit |
| MPEG-4 (Xvid / DivX) | Inter-frame MPEG-4 Part 2 | General-purpose AVI playback, broad compatibility | Default Xvid is the most universally playable AVI codec on Windows |
| H.264 | Inter-frame AVC | Smallest size for shareable copies | Works in AVI containers but MP4 is the more native home |
| Uncompressed | None | Bit-exact archival of TIFF stacks | File size grows linearly with frames × resolution × bit depth |
| MS MPEG-4 v2 / WMV2 | Inter-frame | Legacy Windows Media Player workflows | Useful when feeding old corporate or lab playback stations |
Frame rate is determined by Image Duration. Pick 1/24s for 24 fps, 1/30s for 30 fps, 1/60s for 60 fps, or use the longer presets (1s, 2s, 5s) for slideshow-style playback. Note that AVI's RIFF index stores frame timing per-frame, so non-integer rates (like the 1.8943 fps that researchers sometimes need for time-lapse intervals) may be rounded by downstream players — for sub-fps precision, MJPEG with a custom duration is the most predictable choice.
Yes, when Merge images is selected. Multi-page TIFFs store frames as a linked list of Image File Directories (IFDs); the converter walks the chain and writes each page as a successive video frame. If you instead choose Video per image, each input TIFF (even multi-page) becomes its own output clip.
Use MJPEG if you need to scrub frame-by-frame in ImageJ/Fiji, VirtualDub, or scientific analysis tools — every frame is an independent JPEG, so seeks are exact. Use MPEG-4 / Xvid if you want a small, universally playable AVI on Windows. Use H.264 if file size is the priority and your player supports H.264-in-AVI (most modern players do, but MP4 is more native for H.264). Avoid H.264-in-AVI if the file is going into older lab software that expects DV, Xvid, or MJPEG.
Standard AVI codecs encode at 8 bits per channel, so 16-bit microscopy and astrophotography TIFFs are reduced to 8-bit during conversion. For analysis pipelines that depend on the full bit depth, work from the original TIFFs in ImageJ/Fiji and treat the AVI as a presentation copy. If you must keep high bit depth, look at MOV with ProRes or a 10-bit H.265 MP4 — see TIFF to MP4 or TIFF to MOV.
AVI containers don't compress as efficiently as MP4 by default, and MJPEG (a common AVI codec) stores every frame as a standalone JPEG with no inter-frame prediction. For a smaller file, switch to MPEG-4 (Xvid) or H.264, lower the Quality Preset from Very High to High or Medium, scale the Resolution Preset down (a 4K-resolution time-lapse rarely needs to stay at 4K for review), or shorten the per-frame Image Duration so the same content takes less wall-clock playback time.
This converter writes a silent AVI (image sequences have no native audio track). To overlay a soundtrack, run the AVI through a desktop NLE (Premiere, Resolve, Shotcut) or use Video Cutter to trim it first, then mux in audio. AVI supports PCM, MP3, and AC3 audio at the container level.
A standard AVI with MPEG-4 (Xvid) or MJPEG video plays in Windows Media Player on Windows 10/11 out of the box. Apple removed legacy codec support from QuickTime X (macOS), so for Mac users we recommend an MP4 output instead — see TIFF to MP4. VLC plays everything regardless of OS.
Outputs are written as AVI 2.0 with OpenDML extensions (introduced 1996, led by Matrox), which raises the effective single-file cap from AVI 1.0's ~1 GB practical limit to 4 GB+ via multi-segment RIFF lists. For very long microscopy or astro sequences, that's enough to hold tens of thousands of frames at 1080p MJPEG.
BigTIFF (64-bit offsets, files past 4 GB) is supported on upload. OME-TIFF — the multi-dimensional microscopy variant — converts on the spatial frames; channel and Z-stack metadata is not carried into AVI since AVI has no equivalent dimension. TIFFs using LZW, Deflate, JPEG, PackBits, ZSTD, and WebP internal compression all decode normally before re-encoding into the AVI video stream.